Zheng X.
ASMS 2015 Poster Note 64397
Purpose: To characterize mAbs and ADCs with high resolution Orbitrap mass spectrometer.
Methods: Coupling High Performance Liquid Chromatography with high resolution Orbitrap mass spectrometer (HPLC-MS) enables accurate mass measurement of mAb, ADCs, and peptide mapping experiments.
Results: For Herceptin, 7 major glycoforms were detected; 100% sequence coverage of the ADC sample was achieved, and the modifications, including the drug-conjugated site information were determined; K42 and K103 were 100% modified by DM1 with good reproducibility were observed.
Thermo Fisher Scientific
United States
