This study describes HPAE-PAD methods for the determination of carbohydrates in extracts from instant coffee and green coffee beans. Two methods (the AOAC official method 995.13 and a fast method using the Dionex CarboPac SA10 column) were compared. The former method has a longer run time (80 min) compared to the fast method (10 min). For certain sugars that might be difficult to resolve with the official method, minor modifications are suggested. The fast method, proposed for determining coffee carbohydrates, resolves 7 of the 11 coffee carbohydrates in 8 min (two additional peaks are coelutions of two pairs of carbohydrates) and needs only the addition of DI water for continuous operation.
United States
