Balancing Speed, Sample Complexity and Data Quality in Proteomics Applications on a benchtop Quadrupole-Orbitrap mass spectrometer

[Orbitrap_SciLib]

Arrey T, Damoc E, Xuan Y, Strupat K, Moehring T, Kellmann M.
ASMS 2015 Poster
Purpose: Optimized settings to maximize protein identifications in shotgun analysis using a Thermo ScientificTM Q Exactive HFTM mass spectrometer. Methods: Data dependent MS/MS bottom-up proteomics. Results: A balance in accumulation time, sample complexity/concentration and gradient length is a key achieving maximum peptide/protein identification.


Thermo Fisher Scientific