on 06-07-201310:25 AM - edited on 10-15-202110:20 AM by AnalyteGuru
Canterbury J, Remes P, Senko M, Denisov E, Makarov A ASMS 2013 Poster Purpose: Demonstrate effective automatic calibration and optimization of a variety of
important parameters related to the Thermo Scientific™ Orbitrap™ mass analyzer
Methods: An evolutionary algorithm is employed for optimizing several important ion
transmission parameters simultaneously. Simple one-device optimization curves are
used when one parameter is known to have a dramatic influence on the results of the
optimization. We also show how a novel scoring metric can help alleviate the
complexity of the optimization curve for isotope ratios in particular.
Results: We show that using a multi-dimensional optimization algorithm in conjunction
with appropriately chosen scoring metrics can effectively and reliably optimize
transmission of ions into an Orbitrap mass analyzer, resulting in good isotope ratios
without compromising signal-to-noise.