Pan K, Bomgarden RD, Rogers JC, Viner RI, Meng T, Khoo K.
ASMS 2012 Poster
Novel cysteine-reactive tandem mass tags were developed (IodoTMT, Thermo Fisher Scientific). Quantitative accuracy and precision using IodoTMT were demonstrated. The commercially available anti-TMT resin provides highly efficient enrichment of IodoTMTlabeled peptides. First-ever simultaneous MS-based quantitative analysis of redox-related Cys modifications was achieved by integrating the isobaric IodoTMT tags with sequential alkylating switch.
Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan.
United States
