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Applying Novel Cysteine-reactive Tandem Mass Tags to Quantitative Proteomic Analysis of Reversible Redox Cysteine Modifications

Reputable Mentor II
Reputable Mentor II
Pan K, Bomgarden RD, Rogers JC, Viner RI, Meng T, Khoo K.
ASMS 2012 Poster
Novel cysteine-reactive tandem mass tags were developed (IodoTMT, Thermo Fisher Scientific). Quantitative accuracy and precision using IodoTMT were demonstrated. The commercially available anti-TMT resin provides highly efficient enrichment of IodoTMTlabeled peptides. First-ever simultaneous MS-based quantitative analysis of redox-related Cys modifications was achieved by integrating the isobaric IodoTMT tags with sequential alkylating switch.

Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan.
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‎10-15-2021 11:01 AM
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