Applying H-SRM for the Assay of Midazolam and 1-Hydroxymidazolam in Plasma on the TSQ Quantum Ultra

[steve-day]

This application report presents H-SRM data for the
quantitation of midazolam and its primary metabolite
in protein precipitated plasma. With the selectivity of
H-SRM, sample cleanup not was required (e.g., solid
phase extraction) and a short LC method was sufficient to
accurately quantitate these pharmaceuticals at the level of
10 pg/mL on the Thermo Scientific TSQ Quantum Ultra.