This application proof note demonstrates the determinations of glucose,
fructose, and sucrose in a 100-fold diluted alcoholic beverage sample by
HPAE-PAD. Typically, samples with g/L concentrations require greater than
10,000-fold dilutions to remain in the linear range of the very sensitive
HPAE-PAD technique. However, in this proof note, the method is performed
using a Thermo Scientific
™
Dionex
™
Integrion
™
RFIC
™
system eqipped with a
0.4 μL internal injection loop and the Thermo Scientific
™
High Concentration
Carbohydrate Analysis Kit
1
to extend the linearity from low mg/L to g/L
concentrations. A wood hydrolysate application using this technique is
available for reference.
2