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Reputable Mentor II
Reputable Mentor II
Gingras AC, Gstaiger M, Raught B, Aebersold R.
Nat Rev Mol Cell Biol. 2007 Aug;8(8):645-54.
The versatile combination of affinity purification and mass spectrometry (AP-MS) has recently been applied to the detailed characterization of many protein complexes and large protein-interaction networks. The combination of AP-MS with other techniques, such as biochemical fractionation, intact mass measurement and chemical crosslinking, can help to decipher the supramolecular organization of protein complexes. AP-MS can also be combined with quantitative proteomics approaches to better understand the dynamics of protein-complex assembly.

http://www.nature.com/nrm/journal/v8/n8/full/nrm2208.html
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Ave, Room 992A, Toronto, Ontario, M5G 1X5, Canada.
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