on 06-18-201209:07 AM - edited on 10-15-202111:57 AM by AnalyteGuru
Gingras AC, Gstaiger M, Raught B, Aebersold R. Nat Rev Mol Cell Biol. 2007 Aug;8(8):645-54. The versatile combination of affinity purification and mass spectrometry (AP-MS) has recently been applied to the detailed characterization of many protein complexes and large protein-interaction networks. The combination of AP-MS with other techniques, such as biochemical fractionation, intact mass measurement and chemical crosslinking, can help to decipher the supramolecular organization of protein complexes. AP-MS can also be combined with quantitative proteomics approaches to better understand the dynamics of protein-complex assembly.