on 02-12-201301:55 AM - edited on 10-15-202109:09 AM by AnalyteGuru
Pashmi J, Li S, Kou B, Casteel D. Scientific Poster Purpose: To study the dynamics of the PKGIβ regulatory domain when bound to saturating amounts (1mM) of cGMP or cAMP.
Methods: HDX-MS performed on the PKGIβ peptides in presence of cGMP or cAMP
Results: New instrumentation provided higher coverage (326 peptides vs. 117) using much less protein (3 μg vs. 50 μg) compared to previously acquired results. The increased coverage includes H/DX exchange information for the heretofore unknown leucine zipper region.