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claudia-bouman
Team TFS
Team TFS
1) University of Oxford, Research Laboratory for Archaeology, Dyson Perrins Building, South Parks Road, Oxford OX1 3QY, UK, 2) Thermo Electron Corporation, Bremen, Germany
Rapid Communications in Mass Spectrometry (2006), V20, pp2761–2768, doi: 10.1002/rcm.2651
Analysis of amino acid 13C abundance from human and faunal bone collagen using liquid chromatography/isotope ratio mass spectrometry
James S. O. McCullagh (1), Dieter Juchelka (2) and Robert E. M. Hedges (1)
The scope of compound-specific stable isotope analysis has recently been increased with the development of the LC IsoLink which interfaces high-performance liquid chromatography (HPLC) and isotope ratio mass spectrometry (IRMS) to provide online LC/IRMS. This enables isotopic measurement of non-volatile compounds previously not amenable to compound-specific analysis or requiring substantial modification for gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS), which results in reduced precision. Amino acids are an example of such compounds.We present a new chromatographic method for the HPLC separation of underivatized amino acids using an acidic, aqueous mobile phase in conjunction with a mixed-mode stationary phase that can be interfaced with the LC IsoLink for compound-specific ?13C analysis.
  • IRMS
  • HPLC
  • Carbon isotopes
  • Amino acids
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