Saba J, Drake P, Prakobphol A, Robinson S, Fisher SJ, Viner R.
Scientific Poster
Purpose: To develop a simple and reliable sample preparation method for the study of
sulfated O-linked glycans by employing 2,5-dihydroxybenzoic acid/N,N-dimethylaniline
(DHB/DMA) matrix and MALDI LTQ Orbitrap™ XL hybrid mass spectrometer.
Methods: Sulfated O-linked glycans from mucin were analyzed using DHB/DMA and
DHB matrix on a MALDI LTQ Orbitrap XL mass spectrometer. Pulsed-Q dissociation
(PQD) and collision-induced dissociation (CID) MSn was conducted in the LTQ ion trap for ions of sufficient abundance with detection in the ion trap or Orbitrap detector.
Results: The DHB/DMA matrix enhanced the detection of sulfated O-linked glycans in
the negative mode as compared to traditional DHB matrix. The high mass accuracy and
mass resolution provided by the Orbitrap detector enabled confident structural discrimination between phosphorylated and sulfated species without the aid of tandem
MS. FTMS PQD analysis confirmed the structural composition and enabled site localization of the sulfated group.
Thermo Fisher Scientific
United States
