Hao Z, Bailey D, Russell J, Lee L, McAlister G, Westphall M, Coon JJ, Hühmer A.
ASMS 2011 Poster
Purpose: To investigate the utility of negative ion electron transfer dissociation on the
analysis of small interfering RNA.
Methods: Negative ion electron transfer dissociation was implemented in a hybrid
linear ion trap / Orbitrap™ mass spectrometer under the instrument control software.
Synthetic double stranded siRNA used in this study are from Dharmacon.
Results: Each single siRNA strand fragmented well with negative ion electron transfer dissociation (NETD) and produced primarily a, d, w, type of ions with predominant
peaks being charge reduced precursor species. Fragmentation efficiency of NETD is
relatively low compared to CID or HCD. However, even with low fragmentation
efficiency, close to complete sequence coverage was obtained using a single experimental condition. For all the siRNA strand used in this study, NETD spectrum is less complex compared to CID or HCD spectrum, yet still includes enough information
for close to complete sequence coverage.
Thermo Fisher Scientific
United States
