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Analysis of Small Inhibitory RNA (siRNA) Using Negative Ion Electron Transfer Dissociation in a Linear Ion Trap

Reputable Mentor II
Reputable Mentor II
Hao Z, Bailey D, Russell J, Lee L, McAlister G, Westphall M, Coon JJ, Hühmer A.
ASMS 2011 Poster
Purpose: To investigate the utility of negative ion electron transfer dissociation on the analysis of small interfering RNA. Methods: Negative ion electron transfer dissociation was implemented in a hybrid linear ion trap / Orbitrap™ mass spectrometer under the instrument control software. Synthetic double stranded siRNA used in this study are from Dharmacon. Results: Each single siRNA strand fragmented well with negative ion electron transfer dissociation (NETD) and produced primarily a, d, w, type of ions with predominant peaks being charge reduced precursor species. Fragmentation efficiency of NETD is relatively low compared to CID or HCD. However, even with low fragmentation efficiency, close to complete sequence coverage was obtained using a single experimental condition. For all the siRNA strand used in this study, NETD spectrum is less complex compared to CID or HCD spectrum, yet still includes enough information for close to complete sequence coverage.

Thermo Fisher Scientific
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Last update:
‎10-15-2021 11:51 AM
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