on 06-13-201404:43 AM - edited on 10-15-202107:44 AM by AnalyteGuru
Lin S, Wang H, Hao Z, and Liu X Application Note 20940 The Thermo Scientific™ MAbPac™ SEC-1 column uses size-exclusion chromatography (SEC) and is designed for monoclonal antibody (mAb) analysis, including monomers, aggregates, and fragments. Coupling size-exclusion chromatography with a high-resolution Thermo Scientific™ Exactive™ Plus EMR Orbitrap™ mass spectrometer (SEC-MS) enables accurate mass measurement of mAb and its fragments in either native or denatured state. When using a non-denaturing volatile eluent, such as 20 mM ammonium formate, the intact mass of mAb can be measured in its native state. When using denaturing volatile eluents, such as 20% acetonitrile, 0.1% formic acid and 0.05% trifluoroacetic acid, mAb heavy chain (HC), light chain (LC), Fab, and Fc fragments are successfully separated on MAbPac SEC-1 and their masses are determined accurately.