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Analysis of Monoclonal Antibodies and Their Fragments by Size-Exclusion Chromatography Coupled with an Orbitrap Mass Spectrometer

Reputable Mentor II
Reputable Mentor II
Lin S, Wang H, Hao Z, and Liu X
Application Note 20940
The Thermo Scientific™ MAbPac™ SEC-1 column uses size-exclusion chromatography (SEC) and is designed for monoclonal antibody (mAb) analysis, including monomers, aggregates, and fragments. Coupling size-exclusion chromatography with a high-resolution Thermo Scientific™ Exactive™ Plus EMR Orbitrap™ mass spectrometer (SEC-MS) enables accurate mass measurement of mAb and its fragments in either native or denatured state. When using a non-denaturing volatile eluent, such as 20 mM ammonium formate, the intact mass of mAb can be measured in its native state. When using denaturing volatile eluents, such as 20% acetonitrile, 0.1% formic acid and 0.05% trifluoroacetic acid, mAb heavy chain (HC), light chain (LC), Fab, and Fc fragments are successfully separated on MAbPac SEC-1 and their masses are determined accurately.

Thermo Fisher Scientific
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Last update:
‎10-15-2021 07:44 AM
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