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Orbitrap_SciLib
Reputable Mentor II
Reputable Mentor II
Lin S, Wang H, Hao Z, Horn D, Tracy M, Liu X.
ASMS 2014 Poster
Purpose: Demonstrate SEC-MS as a characterization platform for a monoclonal antibody (mAbs) and its fragments. Methods: Coupling size-exclusion chromatography with high resolution Orbitrap mass spectrometer (SEC-MS) enables accurate mass measurement of mAb, its aggregates, and its fragments. Results: 1. mAb monomer and dimer aggregate intact masses can be measured by SEC-MS under non-denaturing condition using near neutral pH eluent 2. Thermo Scientific™ Exactive Plus™ EMR Orbitrap mass spectrometer enables the accurate detection of mAb at m/z 350- 20,000 3. Thermo Scientific™ MAbPac™ SEC-1 column successfully separates the HC and LC, and partially separates Fab and Fc fragments using denaturing eluent.


Thermo Fisher Scientific
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Last update:
‎10-15-2021 12:05 PM
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