on 06-14-201401:37 AM - edited on 10-15-202112:05 PM by AnalyteGuru
Lin S, Wang H, Hao Z, Horn D, Tracy M, Liu X. ASMS 2014 Poster Purpose: Demonstrate SEC-MS as a characterization platform for a monoclonal
antibody (mAbs) and its fragments.
Methods: Coupling size-exclusion chromatography with high resolution Orbitrap mass spectrometer (SEC-MS) enables accurate mass measurement of mAb, its aggregates, and its fragments.
1. mAb monomer and dimer aggregate intact masses can be measured by SEC-MS
under non-denaturing condition using near neutral pH eluent
2. Thermo Scientific™ Exactive Plus™ EMR Orbitrap mass spectrometer enables the accurate detection of mAb at m/z 350- 20,000
3. Thermo Scientific™ MAbPac™ SEC-1 column successfully separates the HC and LC, and partially separates Fab and Fc fragments using denaturing eluent.