on 05-01-201210:33 AM - edited on 10-15-202107:19 AM by AnalyteGuru
Shi Y, Lafontaine C, Berube M, Fink J, Espourteille F. Application Note 560 Sudan dyes are red dyes used for coloring solvents, oils, waxes, petrol, or as additives in shoe and floor polish. In addition, they have been found in a number of food products such as chili or chili-containing products. Sudan dyes are banned as food additives in the USA, the EU, and many other countries due to links to cancer and other negative health effects. Liquid chromatography-ultraviolet-visible (LC–UV–vis) and liquid chromatography-mass spectrometry (LC/MS) are currently the most popular methods for analysis of Sudan dyes. Traditional sample preparation methods, especially solid phase extraction (SPE), have also been widely used in the determination of Sudan dyes. However, these procedures can be labor-intensive, time-consuming and costly, resulting in low sample throughput when performed manually. Lower recoveries have also been noticed associated with SPE cleanup. There is consensus that one of the major scientific challenges in the analysis of Sudan dyes is to achieve high sensitivity and selectivity while minimizing sample clean up.
In this study we describe an easy, comprehensive LC method using a Thermo Scientific Transcend TLX-1 system powered by TurboFlowTM technology coupled to a Thermo Scientific Exactive MS to analyze five illegal dye residues in a variety of sauces.