This application note demonstrates the analysis of human IgG glycans labeled with a fluorescent tag (2-aminobenzamide) by hydrophilic interaction liquid chromatography (HILIC). The separation is carried out with a Thermo Scientific™ Accucore™ 150-Amide-HILIC 150 Å pore diameter solid core HPLC column. The method displays excellent separation at backpressures compatible with conventional HPLC systems.
United States
