Glycosylation of Asn, Ser or Thr is arguably the most common known post-translational modification (PTM), resulting in a multitude of highly heterogeneous protein isoforms. While the physicochemical differences among the glycosylated protein molecules are often minute, their characterization remains a great analytical challenge. To date, although there are reports of glycoproteome analyses based on electrophoresis techniques, an LC-MS/MS based approach offers advantages in speed, sensitivity, and automation.
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