GRANT APPLICATION RESOURCE
Multichannel, isobaric tagging offers confident and reliable quantitation information for biological experiments. The multiplexing potential of the Thermo Scientific™ TMT10plex™ Isobaric Mass Tag Labelling Kit (Proteome Sciences,® commercially available from Thermo Fisher Scientific) is fully maximized when used with a SPS MS3-based method exclusive to Thermo Scientific™ Tribrid™ mass spectrometers. Uniquely designed Tribrid MS systems are capable of performing synchronous precursor selection (SPS) multi-notch MS3 quantitative analyses to improve experimental throughput and yet still providing the depth and coverage, eliminating issues encountered with TMT ratio distortions caused by interfering ions, without neglecting sensitivity that is often observed. Other commercially available mass analyzers have limitations to handle highly multiplexed isobaric tags as they lack the analytical performance features, i.e. high resolution and multiple analyzers for concurrent isolation and detection of ions, to accomplish a SPS MS3-type technique for quantitation.
United States
