This Application Note updates the work that was done in Thermo Scientific Application Note 313 (AN 313) for the extraction of PAHs from environmental samples. A Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor system was used for the extraction and Thermo Scientific™ ISQ™ Single Quadrupole GC-MS system to analyze 16 PAHs from soil.