on 06-04-201507:49 AM - edited on 10-15-202107:10 AM by AnalyteGuru
Kuehn A, Xuan Y, Tham K, Mueller M, Grosse-Coosmann F, Lange O, Kellmann M, Ueckert T, Henrich C. ASMS 2015 Poster Note 64428 Purpose: The .NET based Application Programming Interface (API) for Thermo Scientific™ Orbitrap™ benchtop mass spectrometer should be used to apply Inter and Intra Run logic decisions during and between LC-MS/MS runs of complex proteomics samples to access optimization strategies of iterative analysis steps.
Methods: The Exactive Series API is used to monitor precursor ion streams at runtime to track the ion flux behavior of potential targets of interest over separation run time level at different LC gradient programs and sample loads. Additionally the API is used to apply intra-run as well as inter-run strategies, for a stepwise performing of iterative LC-MS/MS
Results: Information on the availability of precursors over LC-MS/MS runs can be used at least to adapt the sample load and maximum allowed ion inject times for iterative LCMS/MS runs to stay at a high sequenceing speed with a maximized number of selectable precursors.