A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches.

[Orbitrap_SciLib]

Gunnigle E, Ramond JB, Frossard A, Seeley M, Cowan D.
J Microbiol Methods. 2014 Aug;103:118-23. doi: 10.1016/j.mimet.2014.06.004. Epub 2014 Jun 11.
A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction.

http://www.sciencedirect.com/science/article/pii/S0167701214001596
University of Pretoria, Gobabeb Research and Training Centre