Aaron M. Robitaille1, Jae Choi2, Rosa Viner1, Andreas Huhmer1, Kay Opperman2, John C. Rogers2
TECHNICAL NOTE 72968
Quantitative proteomics strategies using Tandem Mass Tag™ (TMT™) reagents enable sample multiplexing and precise measurement of protein abundance. However, successful execution of this workflow includes multiple steps that may require optimization including liquid chromatography (LC), mass spectrometry (MS), and data analysis. Co-isolated ion interference can suppress accurate ratio quantification and thereby mask true differences in protein abundance across biological systems. Therefore, to assess the accuracy and sensitivity of TMT quantification methods, we developed the Thermo Scientific™ Pierce™ TMT11plex Yeast Digest Standard. Here we demonstrate how the TMT11plex Yeast Digest Standard provides users a tool to measure the precision, accuracy and dynamic range of different mass spectrometry approaches, while also functioning as an excellent quality control assay to assess the LC and MS instrument status.
1 Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, CA 95134.
2 Thermo Fisher Scientific, 3747 N Meridian Rd, Rockford, IL 61101.