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Team TFS
Team TFS
A novel methylation derivatization method for δ18O analysis of individual carbohydrates by gas chromatography/pyrolysis– isotope ratio mass spectrometry
Marco M. Lehmann (1), Maria Fischer (2), Jan Blees (1), Michael Zech (3), Rolf T. W. Siegwolf (1) and Matthias Saurer (1)
1) Laboratory of Atmospheric Chemistry, Paul Scherrer Institute, PSI, CH-5232 Villigen, Switzerland, 2) Laboratory of Nanoscale Materials Science, Empa, Überlandstrasse 129, CH-8600 Dübendorf, Switzerland, 3) Department of Soil Biogeochemistry, Martin-Luther-University Halle Wittenberg, Von-Seckendorff-Platz 3, D-06120 Halle, Germany
Rapid Communications in Mass Spectrometry (2016), V30, pp221–229, doi: 10.1002/rcm.7431
The oxygen isotope ratio (δ18O) of carbohydrates derived from animals, plants, sediments, and soils provides important information about biochemical and physiological processes, past environmental conditions, and geographical origins, which are otherwise not available. Nowadays, δ18O analyses are often performed on carbohydrate bulk material, while compound-specific δ18O analyses remain challenging and methods for a wide range of individual carbohydrates are rare. To improve the δ18O analysis of individual carbohydrates by gas chromatography/pyrolysis–isotope ratio mass spectrometry (GC/Pyr-IRMS) we developed a new methylation derivatization method. Carbohydrates were fully methylated within 24 h in an easy-to-handle one-pot reaction in acetonitrile, using silver oxide as proton acceptor, methyl iodide as methyl group carrier, and dimethyl sulfide as catalyst.
  • Gas Chromatography
  • Oxygen isotopes
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