on 08-14-201202:31 AM - edited on 10-15-202106:34 AM by Closed Account
Kristensen AR, Gsponer J, Foster LJ. Nat Methods. 2012 Sep;9(9):907-9. Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements.