A Platform to Identify Endogenous Metabolites Using a Novel High Performance Orbitrap MS and the mzCloud Library

[Orbitrap_SciLib]

Wang J, Peake DA, Mistrik R, Huang Y.
ASMS 2013 Poster Note
Purpose: To demonstrate the capability of the Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer in combination with MSn library for metabolite structural identification. Methods: UHPLC coupled to the Orbitrap Fusion MS to simultaneously perform global profiling and structural elucidation with MSn. Results: Within UHPLC timescale (2-3 second FWHM), >10 cycles can be finished with fast ion trap HCD MS2 for top 11 precursors, each with ion trees of MS2-MS3 (top2)-MS4 (top2).


Thermo Fisher Scientific