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Team TFS
Team TFS
Application Note 20784. MAb charge variants and protein standards with various pI values have been successfully separated using cation exchange chromatography with a linear pH gradient. This linear pH gradient is generated by running a linear pump gradient from 100% buffer A (at pH 5.6) to 100% buffer B (at pH 10.2). Ruggedness testing of this pH gradient on MAbPac SCX-10 columns shows that retention time RSD is less than 0.8% over 300 runs.
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‎10-16-2013 07:40 AM
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