on 07-11-201305:02 AM - edited on 10-15-202107:42 AM by Closed Account
Peake DA, Yokoi Y, Wang J, Huang Y
Purpose: We present a new workflow for high-resolution Thermo Scientific™ Orbitrap™-based mass spectrometers for lipidomics using a model system consisting of a wild-type strain vs. knockout for Co-Q production in yeast.
Methods: Lipids in yeast mitochondria were analyzed by high resolution LC-MS and MS/MS. Lipid Search® software, an MS2 based search using a comprehensive lipid database, was used to identify the lipid species and determine significant differences.
Results: The yeast lipidomics results obtained from the LC/MS data using Lipid Search are comparable to results obtained using infusion lipidomics. We also compared the lipids identified using metabolomics analysis of the same data set – component finding and molecular weight (MW) search for assignment of metabolites and lipids. Due to the complexity of lipid extracts we found that the comprehensive lipid database MS2 search method is superior to the accurate mass based MW search for lipidomics.