A Modified Orbitrap Tribrid Mass Spectrometer with Real Time Search and Advanced Spectral Processing Enhances Multiplexed Proteome Coverage and Quantification Accuracy

[Orbitrap_SciLib]

Aaron M. Robitaille 1, Romain Huguet 1, Derek J. Bailey 1, Graeme McAlister 1, Arne Kreutzmann 2, Daniel Mourad 2, Daniel Lopez-Ferrer 1, Andreas Huhmer 1, Vlad Zabrouskov 1

Isobaric tagging strategies using Thermo Scientific™ Tandem Mass Tags™ (TMT™) are powerful tools for studying how proteins interact and function in biological systems. Up to 11 samples can be multiplexing in a single high-resolution LC/MS experiment to enable state-of-the-art quantitative analysis of peptide and protein abundance. Here we evaluate the benefits of the Thermo Scientific™ Orbitrap Eclipse ™ Tribrid ™ mass spectrometer including real time search capabilities, advanced spectral processing algorithms, and modified hardware to enhance TMT quantification accuracy and proteome coverage.


1 Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, CA 95134. 2 Thermo Fisher Scientific Hanna-Kunath-Straße 11, 28199 Bremen, Germany