A Mixed-Mode Stationary Phase for the Structural Analysis of Labelled N-Glycans Using LC–MS–MS

[Orbitrap_SciLib]

Aich U, Saba J, Liu X, Rao S, Agroskin Y, Pohl C
The Column. 2013 Dec 4;9(22):32-35
This article describes the analysis of fluorescently labelled N-glycans released from proteins by liquid chromatography–mass spectrometry (LC–MS). The column for this separation used mixed-mode surface chemistry that combines weak anion-exchange and hydrophilic interaction liquid chromatography (HILIC) retention mechanisms for high-resolution and high-throughput analysis of glycans. This combination of modes offers unique selectivity that provides separation of glycans based on charge, size, and polarity. MS and MS–MS analyses were performed using a hybrid quadrupole-orbitrap-based mass spectrometer in negative ion mode to provide detailed structural information of N-glycans released from proteins.

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Thermo Fisher Scientific