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Orbitrap_SciLib
Reputable Mentor II
Reputable Mentor II
David Sarracino1, Susan Abbatiello1, Scott Peterman1, Amol Prakash2, Shen Luan1, Claudia Martins3, Mary Blackburn3
ASMS 2017 Poster
Purpose: Create an integrated workflow to automate highly multiplexed SRM method for evaluation for translational proteomics research. Methods: Utilize sample-specific AMRT spectral libraries and UHPLC separations to determine optimal peptide-based surrogates and corresponding LC and SRM parameters for automated experimental method creation. Incorporation of dynamic retention time correction based on spiked PRTC peptide analysis maximizes SRM scheduling. Results: A robust, multiplexed 35-min SRM method monitoring 950 medium- to high-abundant plasma and serum peptides was created, refined, and used to profile differential expression resulting from different whole blood preparation methods.


1 Thermo Fisher Scientific BRIMS, Cambridge, MA 2 Optys Technologies, Boston, MA 3 Thermo Fisher Scientific, San Jose, CA
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Last update:
‎10-15-2021 06:29 AM
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