on 06-20-201705:18 AM - edited on 10-15-202106:29 AM by Closed Account
David Sarracino1, Susan Abbatiello1, Scott Peterman1, Amol Prakash2, Shen Luan1, Claudia Martins3, Mary Blackburn3 ASMS 2017 Poster Purpose: Create an integrated workflow to automate highly multiplexed SRM method for evaluation for translational proteomics research.
Methods: Utilize sample-specific AMRT spectral libraries and UHPLC separations to determine optimal peptide-based surrogates and corresponding LC and SRM parameters for automated experimental method creation. Incorporation of dynamic retention time correction based on spiked PRTC peptide analysis maximizes SRM scheduling.
Results: A robust, multiplexed 35-min SRM method monitoring 950 medium- to high-abundant plasma and serum peptides was created, refined, and used to profile differential expression resulting from different whole blood preparation methods.
1 Thermo Fisher Scientific BRIMS, Cambridge, MA
2 Optys Technologies, Boston, MA
3 Thermo Fisher Scientific, San Jose, CA