Peake D, Kiyonami R, Yokoi Y, Fukamachi Y, Huang Y.
ASMS 2015 Poster Note 64485
Purpose: To demonstrate a High Resolution Accurate Mass (HRAM) approach for the quantitation of Buprenorphine and Paroxetine in the presence of protein precipitated rat plasma.
Methods: Sample analysis was performed using Single Ion Monitoring (SIM) with generic mass spectrometer source conditions and chromatographic separation utilizing ultra high performance chromatography (UHPLC).
Results: Sample analysis demonstrated a lower limit of quantitation (LOQ) of 10pg/mL for both analytes, a linear signal response of more than four orders of magnitude, a coefficient of variance of less than ten percent for all replicate injections.
Thermo Fisher Scientific and Mitsui Knowledge Industry
United States
