μPAC Neo HPLC columns: deeper proteomic coverage with shorter runtime

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Ultrasensitive MS-based proteomics has seen remarkable breakthroughs in recent years. Improvements at different stages in the proteomics workflow have pushed sensitivity to a level that now allows scientists to successfully identify and quantify more than 1,000 proteins from a single mammalian cell. 

μPAC Neo HPLC columns.Optimized sample handling, maximum separation efficiency at low nanoLC flow rates and high-resolution mass spectrometry are key technologies to achieve maximum sensitivity. Increase in sampling depth and proteome coverage can be accomplished by rapid advances in mass spectrometer technology. However, appropriate selection of the nanoLC column is critical to provide MS instruments with peptides separation at the highest possible resolution. 

Meet the μPAC Neo HPLC columns 

Thermo Scientific™ μPAC™ (micro Pillar Array Column) technology is unique — built by precise micromachining chromatographic separation beds from silicon. This approach brings three critical and unique characteristics:    

• Perfectly ordered flow path – Thermo Scientific μPAC Neo HPLC Columns are designed with a perfect order, eliminating heterogenous flow paths. The ordered flow path of the μPAC columns minimizes the dispersion to the overall separation resulting in sharper and more intense chromatographic peaks. 
• Column-to-column reproducibility – Each column is manufactured using the same lithographic mask. The unique micromachining manufacturing results in columns that are virtually identical. The increased injection-to-injection and column-to-column reproducibility provides increased confidence in results throughout the duration of a research study. 
• Performance and robustness – μPAC columns operate at moderate pressure, allowing longer flow paths for increased separation performance and sensitivity. Operating at lower back pressures also allows for a longer column lifetime. 

The column can be operated at moderate LC pump pressures up to 450 bar over a wide range of flow rates: 

• 50 cm μPAC Neo LC column: 0.1-0.75 μL/min 
• 110 cm μPAC Neo LC column: 0.1-0.75 μL/min 
• 50 cm low-load μPAC Neo LC column: 0.1-0.75 μL/min 

Want to learn more? Find more product details on this web page 

Additional Resources

• μPAC Neo product spec sheet  
• Proteomic product flyer  
• Poster note: High aspect ratio pillar array columns for deep proteome profiling at moderate LC pump pressures 
• Poster note: Advancing nanoLC-MS sensitivity for single cell proteomics using solid silicon micro-pillar array column technology