Can we use BIopharma Finder to do RNase mapping to distinguish circRNA from it's precursor RNA?

[zhounan919]

Can we use BIopharma Finder  to do RNase mapping to distinguish circRNA from it's precursor RNA?

Thanks

@IofEyes1

[Molly_I2]

@zhounan919 thanks for your question. I will find an answer for you and follow up!

[RobertRoss]

Hi,

RNase mapping using the partial digestion strategy will produce relatively longer oligonucleotides that can be matched against your RNA sequence. In your digestion, if the template which is used to create the circular RNA has a different sequence (complementary to the circular RNA) and you have this precursor sequence as well as the product strand sequence entered into your processing experimental parameters, then yes, BPF will be able to distinguish the two. 

If you would like to discuss further, feel free to reach out at robert.ross2@thermofisher.com.