Thanks for sharing. Yes, the topic of comprehensive analysis of compounds in metabolomics is a current and challenging topic still . Given the chemical diversity of compounds, selecting a separation/chemistry technique , whether it be LC (RP or HILIC) , IC, or GC already means you have biased your study towards those compounds amenable to that mode of separation . So in the example above, if you focus on a target class of compounds , then you have to think of a technique that gives you the strongest retention/separation , such as the primary metabolites in this example . If you are after as much unbiased coverage as possible, then you may need to choose multiple separation modes such as RP and HILIC if you are using LC . Still an ongoing challenge in the community .
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