Showing results for 
Search instead for 
Did you mean: 

Incorporating lock masses with Q Exactive HF

Involved Contributor II
Involved Contributor II

We perform routine mass calibrations on our Q Exactive HF system--at least every five days but often more frequently--but do not typically incorporate lock masses in our methods. In an analysis run on a calibration day, we can achieve acceptable mass accuracies so we have not felt the need to incorporate lock masses. However, we don't want to miss out on the opportunity to improve our methods! How, then, do other users incorporate lock masses, if at all, and how does that relate to your calibration schedule (i.e. do you perform external calibration at the beginning of the week and then use lock masses to correct drift over the week)?


Community Manager
Community Manager

Hi @mnewmeyer and welcome to our online community. We will work to get some insight provided for you on this great question and appreciate you sharing it in the forum. @FourierTFormer and @shannon_eliuk - Please help me say hello!

Team TFS
Team TFS

Hi @mnewmeyer  and welcome to our online community. I am an application chemist working both in the field and in the demo lab.  Lock mass correction can be used in a method to help account for any mass drift seen over time since the last calibration, and many customers  utilize it. There are also other customers who do not use a lock mass, and find the orbitrap to meet their accuracy needs, with good success like you. The QE HF is capable of 3 ppm RMS under defined conditions with external calibration and can do better with a lock mass.  I will connect with you directly, and we can discuss some of my personal experiences in more depth once I get a better understanding of the type of work you are using your orbitrap for.




mzCloud - A State of the Art Mass Spectral Library

Some resources only sound too good to be true.

mzCloud is a state of the art mass spectral database that assists analysts in identifying compounds.
It is a freely searchable collection of high resolution accurate mass spectra that can be accessed online free of charge.

Try it today!