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How does an adjustable gradient delay volume (GDV) in an HPLC system help with method transfer?

Team TFS
Team TFS

The transfer of an existing HPLC method from the one HPLC instrument to a new instrument, can be a challenging task for chromatographers. 😣

Differences in the hardware designs between two instruments can affect chromatographic results.

For gradient HPLC methods the instrument GDV, is defined as the volume from the point of mixing of the eluents to the column head and plays a critical role in a successful method transfer. Modifying the GDV is one of the first adjustments chromatographers should consider when transferring a method between two different HPLC systems.

There are several ways that users adjust the GDV; indirectly by using isocratic holds or using software to time-shift the injection which might change the elution behavior or analytes which may require re-validating the method; or directly by physically changing fluidic components of the flow path by exchanging mixers or capillary tubing, which may require the user to re-qualify or re-validate the system.


Did you know there is an easier way to transfer methods between two HPLC systems without re-validating or re-qualifying an instrument?

The Thermo Scientific Vanquish Core HPLC system features an integrated metering device as part of the fluidic pathway, which enables users to freely tune and adjust the gradient delay volume in 1 µL increments from 0-230 µL, an optional method transfer kit can be added to adjust the gradient delay volume up to 430 µL.

Since this tunable feature is built into the system adjustment of the metering device means no need to re-plumb or re-qualify the system whenever you need to adjust the GDV – saving you time and extra paperwork to remain compliant in regulated environments.


BA74021-Vanquish-Product-Demo-facebook-banner-1200x628.jpg➡️Learn more about the adjustable GDV feature in the Vanquish Core and check out the virtual demo at 








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