Due to the small sample size and extensive sample preparation needed, sensitivity and sample utilization are two main challenges for clumped isotopes analysis.
When it comes to the system setup for performing the clumped isotope analysis, stable lab environment during the measurement with variations as small as ±0.1°C/h is a critical factor. Another challenge is precise matching of the sample and reference capillaries. Any minor mismatch can affect the quality of the data. These challenges can be overcome by using Thermo Scientific LIDI 2 Software Workflow.
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