Analyzing small metabolites is necessary to identify biomarkers of certain disease states. Ion chromatography (IC) is an effective technique for separating small, polar metabolites including the TCA and Glycolysis cycle metabolites. Techniques including reversed–phase chromatography and HILIC lack the required selectivity for such applications. Additionally, High-Resolution Accurate-Mass (HR/ AM) mass spectrometry can confirm the identity of the given metabolite. Learn about how coupling IC with HR/AM mass spectrometry provides better sensitivity and resolving power to determine a large quantity of polar metabolites.