September 2021 saw the launch of a new low-flow UHPLC solution, the Thermo Scientific™ Vanquish™ Neo UHPLC system and Thermo Scientific™ PepMap™ Neo UHPLC columns.
When we talk about HPLC columns, terms such as pore size, reversed-phase, fully porous, and packing media are often used. It leaves the question, how can you be sure the column can do the job you need it to, reliably, and easily?
Designing and developing a new product is an amazing process and I’m fortunate enough to be in a role that allows me to see an idea turn into a prototype, to an alpha, to a beta, and into production. During this process the product and the manufacturing process are ruggedly tested, including asking customers to use the products and provide feedback. We now have collected data and received customer feedback that demonstrates the exceptional performance of these columns.
Who’s behind the column?
It takes a dedicated team to design and manufacture a product. I’d like to introduce you to some of the team who worked on the PepMap Neo Columns and the less well-known aspects of their roles.
Paulius Trumpickas - R&D Engineer: “I was responsible for PepMap Neo column development and transfer to manufacturing. For these columns we introduced an additional test to ensure that the columns can withstand up to 1500 bar pressure.
“In addition to my engineering responsibilities, I am developing software to automatically collect and analyze production data. We recently implemented a data analysis system to carry out daily reliability checks of our column QC equipment. Currently, we are developing software to automatically monitor production data and produce weekly reports. All this contributes to our goal of making the Vilnius column production site a modern, smart factory.“
Runsheng Zheng – Product Specialist: “I focused on developing and publishing low-flow LCMS methods for deep-dive (Technical Note 74152) and high-throughput (Technical Note 000138) proteomics studies. We aimed to provide standardized and optimized methods for different application needs using the Vanquish Neo UHPLC system and PepMap Neo columns. The deep-dive proteomics methods using a 75 µm × 75 cm EASY-Spray column have reproducibly identified more than 80,000 peptides and 7,000 proteins from 1 µg HeLa digest in single-shot experiments. While using the high-throughput methods with a 75 µm × 15 cm EASY-Spray column in the trap-and-elute mode, we enable users to perform large-cohort proteomics sample screening with maximal MS utilization up to 95% and sample throughput up to 180 samples per 24 hours. All of these methods are highly reproducible with different batches of columns among three labs, regardless of multiple sources of variations.”
Chris Pynn – Nano LC Specialist: “For this project to fulfil its potential, the complete solution comprising the LC, the columns and the interface with the MS had to be a complete success. In order to help achieve this, I had the privilege to work closely together with teams of talented people from across the organization, people responsible for every component, from columns and other consumables to software, ion sources and MS systems. Not forgetting my own colleagues, of course, who worked so diligently on the various aspects of LC development in the LC factory to ensure that an easy-to-use and effective instrument user experience remained at the center of the instrument design. Additionally, I have been working with customers from both academia and industry – helping to set new standards in nano, capillary and micro flow LCMS separation science. I have learned a great deal from you all, thank you!”