Rapid, Automated Peptide Mapping for Multi Attribute Monitoring

[michael-oliver]

Peptide mapping is a fundamental workflow required to ensure bio-therapeutics structural integrity is maintained at all stages of the drug development pipeline.  This is required for maintenance of drug efficacy and patient safety.

Current sample preparation techniques utilized for peptide mapping are complex and prone to error; this can have a dramatic effect on reanalysis rates, transfer and implementation of methods at different sites.  There is also the question of how techniques can be developed to prevent or minimize reproducibly Post Translational Modifications (PTMs) especially in relation to Multi Attribute Monitoring.

With the continued success and growth of bio-therapeutics these issues are further compounded by the drive towards automation to facilitate high throughput processing.

This webcast details how new technologies can deliver peptide mapping which is:

• Fast


• Provides high quality results with low Post Translational Modifications (PTMs)


• Easy to automate


• Easy for untrained operators


• Reproducible and transferable

You’ll also learn:

• How new automated technologies compare to existing practices


• The challenges faced in peptide mapping and sample preparation


• How new technologies overcome issues with automation of peptide mapping, including PTMs


• How new automation in 96 well plate format is reproducible, fast and easy to use

About the speakers:

Dr Sara Carillo - Applications development team leader

Dr. Sara Carillo is the applications development team leader in the Characterisation and Comparability Laboratory in NIBRT, Ireland. Dr. Carillo completed her PhD in Chemical Science at the University of Naples in 2013 working on bacterial glyco-conjugates structural characterisation. In 2015 Dr. Carillo joined Dr. Jonathan Bones’s research group in NIBRT, working on CHO cell glycome and biotherapeutics characterisation.

Dr Ken Cook - EU Bio-Separations Expert

Dr. Ken Cook has 30 years of experience supporting liquid and ion chromatography applications with Dionex and then Thermo Fisher Scientific. Dr. Cook currently supports customers with bio-separations, focusing on biotherapeutics. Previously Dr. Cook was a lecturer in biochemistry at the University of Newcastle-Upon-Tyne, UK where he focused on protein biochemistry.