Recently, I was applying for a visa to visit India. I had two options – either I walk my application into the San Francisco Indian Embassy or I could mail in my application and my passport. The number of steps required in the former option for me to get my visa was a bit too much in my opinion. Who wants to drive an hour, find parking in a big city like San Francisco, and then waste time in long lines even when I already scheduled an appointment? With the latter option, I simply had to ship everything off and verify each stage of my application’s journey from the comfort of my pajamas at home.
Similar tradeoffs are made every day even in research laboratories when choosing the right tool that gets you the results you need without sacrificing your entire workday. Addressing issues like:
Improving turnaround time and improving throughput for the research team you serve
Managing every research dollar counts; best cost per selectivity
Accessing technology to drive rich insights across large scale studies
Given the nature of peptide quantitation, you need a tool for method development that can help screen a large quantity of peptides for multiple proteins at a time. Essentially, it’s a tradeoff between tracking a visa application in your pajamas versus taking an entire day to get your visa.
To achieve ultimate sensitivity and selectivity when performing proteomic quantitation, the Thermo Scientific TSQ Altis Triple Quadrupole Mass Spectrometer was designed. One of the key benefits for peptide quantitation is the instrument’s ability to perform large numbers of transitions per unit time without compromising performance. Being able to target more within the same injection is a huge benefit for any user! Imagine you have 100 proteins with five peptides per protein. Each peptide would need five transitions so you are at 2,500 transitions already!
Dr. MacCoss’s protein mass spectrometry lab at the University of Washington, where they use mass spectrometry-based approaches to improve their understanding of biology on a molecular, cellular, and whole organism level, evaluated the Thermo Scientific TSQ Altis mass spectrometer for peptide quantitation. They have used triple quadrupole-based instruments for this application as they find the technique is hard to beat when it comes to cost per selectivity ratio. Upon evaluation of the instrument, three main performance benefits were highlighted:
Increased sensitivity for peptide quantitation
Enables more transitions (with excellent performance) using the same cycle time