Liquid chromatography mass spectrometry (LC-MS) is one of the most powerful and versatile tools available for quantitative and qualitative analysis.
LC-MS users often divide themselves into one of two camps – chromatographer or mass spectrometrist. If you ask a chromatographer about mass spectrometry, they may just refer to it as a detector. If you ask a mass spectrometrist about liquid chromatography, they’ll just consider it the front end to their mass spectrometer. (Which one do you identify with most? Check out this blog post to learn more.)
However, as the name implies there are two parts to consider – liquid chromatography AND mass spectrometry. To get the best performance and data from both instruments, scientists need to focus on both ends – the separation and the detection.
Although liquid chromatography technology plays an essential part in LC-MS analysis, it is often overshadowed by advances in mass spectrometer performance and usability. What’s important to recognize is that without some sort of separation technique — like liquid chromatography — to help separate the sample in time and reduce the interfering matrix, it’s likely impossible that a mass spectrometer alone could get the level of detailed sample analysis for complex samples without it.
Like a covalent bond, it truly takes two to tango.
Lucky for you we just kicked off a six-part podcast series called “How Liquid Chromatography Is Pushing the Boundaries of Low-flow LC-MS Analysis.” In this exclusive series, you’ll understand the crucial role of nano-, capillary-, and micro-low LC separations in proteomics LC-MS applications.